Ed2k-hash

If you have the file(s) on your harddisk or on cd you can use all kinds of tools to generate the ed2k link and other additional info:

AniDB_O'Matic by BennieB/PetriW can generate ed2k links. It generates ed2k link, md5, crc in one go and also lists stuff like codec, resolution, bitrates, ...
ed2k_hash another tool which is commandline based and also available for linux.
Filehash a little java programm written by Malich. for further info on it read here
Hashcalc can create md5, sha-1, crc32, ed2k-links and various other hashes in 1 go. though at least 1 case is known in which it created a wrong ed2k!